Table I.
Effect of Cyclin BΔ90 mRNA Injection on Anaphase Motilities
Type of movement | Sample | Average velocity | Sample number | |||
---|---|---|---|---|---|---|
(μm/min) ±SD | ||||||
Anaphase A | Control | 2.6 ± 1.1 | 10 | |||
chromosomes | cyclin BΔ90 | 2.4 ± 1.0 | 12 | |||
Anaphase B* | Control | 2.8 ± 1.0 | 16 | |||
chromosomes | cyclin BΔ90 | 2.7 ± 0.9 | 15 | |||
Anaphase B | Control | 1.8 ± 0.6 | 8 | |||
poles | cyclin BΔ90 | 1.7 ± 0.5 | 7 |
Anaphase B is determined based on the movement of spindle poles. Note that the poles move at a slower speed than the chromosomes during anaphase B, implying that anaphase A and B do not occur entirely sequentially in these cells.