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. 1997 Jul 28;138(2):271–281. doi: 10.1083/jcb.138.2.271

Figure 1.

Figure 1

Requirements to residue 131 and 132. (A) Schematic representation of the amino acid sequences in the cytoplasmic tails of the CD3γ chains expressed in the indicated cell lines and a summation of the results from the TCR downregulation analyses. TCR downregulation was scored according to the percent anti-CD3 binding after incubation with PDB (110 nM) for 1 h: +++, 0–40% anti-CD3 binding; ++, 40–60% anti-CD3 binding; +, 60– 80% anti-CD3 binding; (+), 80–95% anti-CD3 binding; −, >95% anti-CD3 binding. (B) Cells were incubated with different concentrations of PDB for 1 h and TCR downregulation was determined by staining with anti-CD3 mAb and flow cytometry comparing MFI of PDB-treated cells with MFI of untreated cells. (C) FACS® histograms of untreated cells (white, dotted line) and cells treated with 110 nM PDB (black) for 1 h. The cell line and the percent anti-CD3 binding after PDB treatment are given in the upper left corner of each histogram. The ordinate indicates the relative cell number. The abscissa indicates the fluorescence intensity in a logarithmic scale in arbitrary units. MFI of the cell lines stained with irrelevant mAb varied between two and five arbitrary units (data not shown).