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. 1997 Jul 28;138(2):271–281. doi: 10.1083/jcb.138.2.271

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Binding of AP to mutated CD3γ peptides. (A) Western blot of material from T cell cytosol bound by beads coated with GAM-S126A (lanes 1–3) and GAM-D127A (lanes 4–6) peptides blotted with the anti–α-adaptin antibody. The amount of beads used represented 200 (lanes 1 and 4), 100 (lanes 2 and 5), and 50 (lanes 3 and 6) nmol bound CD3γ peptide. A plot of the bands quantitated by densitometry is given below. The bands are normalized to the band obtained with 200 nmol GAM-WT peptide. (B) Western blot of material from T cell cytosol bound by beads coated with GAM-WT (lanes 1 and 2), GAM-INS (lanes 3 and 4), and GAM-DEL (lanes 5 and 6) peptides blotted with the anti–α-adaptin antibody. The amount of beads used represented 200 (lanes 1, 3, and 5) and 100 (lanes 2, 4, and 6) nmol bound CD3γ peptide.