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. 1997 Jul 28;138(2):271–281. doi: 10.1083/jcb.138.2.271

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Internalization and degradation is reduced by insertion or deletion of amino acids in the DxxxLL motif. (A) Schematic representation of the amino acid sequences in the cytoplasmic tails of the CD4/CD3γ chimeras expressed in the indicated cell lines, and a summation of the results obtained from FACS^® analysis, immunofluorescence microscopy, pulse–chase metabolic labeling, and internalization experiments. IC, intracellular; PM, plasma membrane. (B) The FACS^® profiles show the indicated cell line in black and the JGN cell line in white. (C) Pulse–chase metabolic labeling. JGN-CD4/3-SA, JGN-CD4/3-1A, and JGN-CD4/3-dT130 cells were pulsed for 30 min (lane 1) and chased for 1 (lane 2), 2 (lane 3), and 4 (lane 4) h. (D) Internalization of anti-CD4 antibodies by chimeric receptors. The percentage of internalized anti-CD4 to cell surface bound anti-CD4 was calculated as described in Materials and Methods.