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Amino acid alignment of CD66 N-domains used in this study. Protein sequences were deduced from DNA sequencing of the clones. Dots indicate residues identical to those in CD66e. Arrows indicate the regions corresponding to the primer binding sites for PCR amplification of N-domains from HeLa-CD66 DNA. The location of the Cac8I site present in the DNA used to construct CD66b/e chimeric N-domains is indicated at the corresponding residue 59.
