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. 1998 Aug 4;95(16):9584–9589. doi: 10.1073/pnas.95.16.9584

Figure 2.

Figure 2

Interaction of MS11 Opa variants with CD66e glycoforms. (A) Immunoblot showing binding of CD66e glycoforms by gonococcal Opa variants. CD66e glycoforms were isolated from CD66e-transfected CHO cell lines by using PI-PLC (lanes 1–3). Binding of CD66e glycoforms to Opa− (-) or OpaI (I) expressing gonococci is shown in lanes 4–9. Bound CD66e was detected with anti-CEA rabbit serum. Mr markers (× 10−3) are indicated on the left. The cell types from which CD66e was derived are indicated above the blot. (B) Adherence and entry of OpaI variants to Pro5, Lec2, and Lec8 cells and their CD66e-expressing counterparts. GC, gonococci. Data are means ± SE of three independent experiments.