Figure 6.
Interaction of Opa54/F and Opa59/H variants with CD66e and recombinant CD66e-N-domain. (A) Binding of CD66e, derived from Pro5-CD66e cells through PI-PLC treatment, by recombinant Opa variants and their wild-type counterparts. Bound CD66e was detected with anti-CEA rabbit serum. Below the designation of the recombinant Opa variants the nomenclature of the wild-type Opa homologs is indicated in parentheses. (B) Binding of recombinant CD66e-N-domain by wild-type Opa variants. Opa−, F, H, and I variants were incubated with cleared lysates of E. coli containing the CD66e N-domain and processed for immunoblotting. Bound N-domain was detected with anti-His antibody. Results with Opa60/I are shown as a positive control. The figure is representative of three independent experiments.