Figure 7.

Binding of E. coli-expressed CD66e, CD66e/b, and CD66b/e N-domain chimeras by recombinant MS11 Opa variants. Opa variants were incubated with cleared lysates of E. coli expressing the indicated N-domains and processed for immunoblotting. Bound N-domain was detected with anti-His antibody. The lane designated lys contains only the E. coli lysate. Below the designation of the recombinant Opa variants used the nomenclature for wild-type Opa homologs is indicated in parentheses. The figure is representative of three independent experiments.