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. 1997 Jul 28;138(2):435–448. doi: 10.1083/jcb.138.2.435

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IFN inducibility of NDP52 and Sp100 mRNAs as determined by Northern blot analysis. Total RNA of untreated (lanes labeled ∅), IFN β– (lanes labeled β), and IFN γ– (lanes labeled γ) treated HEp-2 cells was analyzed using radiolabeled full-length NDP52 (lanes labeled NDP52), Sp100 (lanes labeled Sp100), and β-actin (lanes labeled Beta-Actin) cDNAs as probes. The amount of Sp100 mRNA is increased by IFNs β and γ (fifth and sixth lanes, respectively). The NDP52 mRNA level is not significantly increased by IFN β and only weakly by IFN γ (eighth and ninth lanes, respectively).