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. 1997 Jul 28;138(2):435–448. doi: 10.1083/jcb.138.2.435

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Failure of detection of NDP52 autoantibodies in sera from PBC patients containing anti-PML and anti-Sp100 autoantibodies by immunoprecipitation. Radiolabeled NDP52 and, as a negative control, luciferase proteins were mixed and immunoprecipitated using five anti-Sp100/anti-PML double-positive PBC sera (lanes PBC1–PBC5). In lane PBC3, radiolabeled PML was included in the precipitation reaction as a positive control. The first and last lanes represent mixtures of the translation reactions as indicated.