Table I.
In Vivo Interaction Studies Using the Mammalian Two-Hybrid Assay
| pV-NDP | pV-Sp100 | pV-PML | pV-largeT | |||||
|---|---|---|---|---|---|---|---|---|
| pg | ||||||||
| pM-NDP | 290.3 | < | < | ND | ||||
| 296.5 | 1.1 | 1.7 | ||||||
| pM-Sp100 | < | ND | 1.6 | ND | ||||
| 0.4 | 1.8 | |||||||
| pM-PML | < | 2.4 | 28.0 | ND | ||||
| < | 2.5 | 20.2 | ||||||
| pM-53 | ND | ND | ND | 186.4 | ||||
| 251.1 | ||||||||
Values from two representative experiments are given. The Ga14 (pM-) and VP16 (pV-) constructs used in each respective cotransfection can be seen on the left side and on top of the scheme, respectively. Shown are the amounts of CAT protein in picograms in a 200-μl assay volume standardized to equal amounts of β-galactosidase expression. No NDP52 construct gives a signal above background when cotransfected with the PML or the Sp100 constructs. The pV-NDP/pM-NDP combination and the pV-PML/pM-PML combination, however, show a strong or intermediately strong positive signal, respectively, indicating that NDP52, like PML, homodimerizes. <, below detection limit.