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. 1998 Aug 4;95(16):9590–9595. doi: 10.1073/pnas.95.16.9590

Figure 1.

Figure 1

Effect of HPr and HPr-P (Ser-46) on CcpA binding to amyO. Various concentrations of the HPr or HPr-P (Ser-46) (0.68–3.4 μM) were combined with the amount of CcpA required for one-half saturation of amyO (28 nM). After the amyO fragment was added to the binding buffer containing CcpA (C), CcpA + HPr, or CcpA + HPr-P (Ser-46) and allowed to form complexes; DNase I was added to digest unprotected DNA. A/G represents A+G ladder of coding strand of the amyO fragment and the arrow indicates a band showing hypersensitivity to DNase I digestion. The location of the amyO site is indicated by the box on the Left.