Figure 4.

Stimulation of CcpA-mediated inhibition of transcription by the HPr protein (A) and FDP or NADP (B). (A) After various amounts of the HPr or HPr-P (Ser-46) (0.68–10.8 μM) were combined with CcpA (0.18 μM), transcription buffer and template DNA were added. Transcription was started by adding RNAP to the mixture and incubating for 15 min at room temperature. (B) CcpA (0.18 μM) was combined with FDP (1.5–12 mM) or NADP (0.4–1.5 mM) and transcription was performed as described in A. The transcripts from the amyE and veg promoters were labeled on the Left, and lane C (control) in both A and B indicates transcription carried out in the absence of CcpA.