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. 1969 Jun 1;129(6):1183–1201. doi: 10.1084/jem.129.6.1183

REGULATION OF THE IMMUNE RESPONSE

I. REDUCTION IN ABILITY OF SPECIFIC ANTIBODY TO INHIBIT LONG-LASTING IGG IMMUNOLOGICAL PRIMING AFTER REMOVAL OF THE FC FRAGMENT

Nicholas R StC Sinclair 1
PMCID: PMC2138663  PMID: 5305714

Abstract

The ability of 7S and F(ab')2 antibody fragments to suppress priming with low doses of antigen was compared. The 7S preparation was approximately 100–1000 times more potent than the F(ab')2 preparation when the agglutinin titers of the two preparations were the same. The presence of any ability to suppress priming in the F(ab')2 preparation may reflect an inherent capacity of the F(ab')2 antibody or contamination with small amounts of 7S antibody. The difference between 7S and F(ab')2 antibody in ability to suppress priming is attributed to the lack of the Fc portion on the F(ab')2 antibody. The Fc portion may be needed to prevent rapid excretion of antibody from the body, to induce rapid phagocytosis of antigen-antibody complexes with consequent breakdown and elimination of antigen, or to inactivate or suppress the antigen-sensitive cells from reacting to antigenic determinants. More detailed studies will permit a better assessment of the importance of these three possible regulatory roles of the Fc portion of the immunoglobulin in the immune response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelrad M. A. Suppression of delayed hypersensitivity by antigen and antibody. Is a common precursor cell responsible for both delayed hypersensitivity and antibody formation? Immunology. 1968 Aug;15(2):159–171. [PMC free article] [PubMed] [Google Scholar]
  2. Axelrad M., Rowley D. A. Hypersensitivity: specific immunologic suppression of the delayed type. Science. 1968 Jun 28;160(3835):1465–1467. doi: 10.1126/science.160.3835.1465. [DOI] [PubMed] [Google Scholar]
  3. Boyns A. R., Hardwicke J. Immunological responsiveness following the continuous circulation of soluble antigen--antibody complexes. Immunology. 1968 Aug;15(2):263–269. [PMC free article] [PubMed] [Google Scholar]
  4. Dixon F. J., Jacot-Guillarmod H., McConahey P. J. The effect of passively administered antibody on antibody synthesis. J Exp Med. 1967 Jun 1;125(6):1119–1135. doi: 10.1084/jem.125.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Greenbury C. L., Moore D. H. Non-specific antibody-induced suppression of the immune response. Nature. 1968 Aug 3;219(5153):526–527. doi: 10.1038/219526a0. [DOI] [PubMed] [Google Scholar]
  6. James K. Some factors influencing the ability of anti-lymphocytic antibody to suppress humoral antibody formation. Clin Exp Immunol. 1967 Nov;2(6):685–690. [PMC free article] [PubMed] [Google Scholar]
  7. MOLLER G. ANTIBODY-INDUCED DEPRESSION OF THE IMMUNE RESPONSE: A STUDY OF THE MECHANISM IN VARIOUS IMMUNOLOGICAL SYSTEMS. Transplantation. 1964 May;2:405–415. [PubMed] [Google Scholar]
  8. NEIDERS M. E., ROWLEY D. A., FITCH F. W. The sustained suppression of hemolysin response in passively immunized rats. J Immunol. 1962 Jun;88:718–724. [PubMed] [Google Scholar]
  9. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  10. Naysmith J. D., James K. Effect of F(ab')2 from rabbit anti-mouse lymphocyte IgG on the graft versus host reaction in F1 hybrid mice. Nature. 1968 Jan 20;217(5125):260–261. doi: 10.1038/217260a0. [DOI] [PubMed] [Google Scholar]
  11. ROWLEY D. A., FITCH F. W. HOMEOSTASIS OF ANTIBODY FORMATION IN THE ADULT RAT. J Exp Med. 1964 Dec 1;120:987–1005. doi: 10.1084/jem.120.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  13. SPIEGELBERG H. L., WEIGLE W. O. THE CATABOLISM OF HOMOLOGOUS AND HETEROLOGOUS 7S GAMMA GLOBULIN FRAGMENTS. J Exp Med. 1965 Mar 1;121:323–338. doi: 10.1084/jem.121.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. STONER R. D., TERRES G. ENHANCED ANTITOXIN RESPONSES IN IRRADIATED MICE ELICITED BY COMPLEXES OF TETANUS TOXOID AND SPECIFIC ANTIBODY. J Immunol. 1963 Dec;91:761–770. [PubMed] [Google Scholar]
  15. Sinclair N. R. Effects of neonatal thymectomy on the haemolysin response to sheep erythrocytes in Swiss albino mice. A time-course study of total, 19S and 7S antibody. Immunology. 1967 May;12(5):549–557. [PMC free article] [PubMed] [Google Scholar]
  16. Sinclair N. R., Elliott E. V. Neonatal thymectomy and the decrease in antigen-sensitivity of the primary response and immunological "memory" systems. Immunology. 1968 Sep;15(3):325–333. [PMC free article] [PubMed] [Google Scholar]
  17. Sinclair N. R., Lees R. K., Elliott E. V. Role of the Fc fragment in the regulation of the primary immune response. Nature. 1968 Dec 7;220(5171):1048–1049. doi: 10.1038/2201048a0. [DOI] [PubMed] [Google Scholar]
  18. Stuart F. P., Saitoh T., Fitch F. W. Rejection of renal allografts: specific immunologic suppression. Science. 1968 Jun 28;160(3835):1463–1465. doi: 10.1126/science.160.3835.1463. [DOI] [PubMed] [Google Scholar]
  19. TERRES G., WOLINS W. Enhanced sensitization in mice by simultaneous injection of antigen and specific rabbit antiserum. Proc Soc Exp Biol Med. 1959 Oct-Dec;102:632–635. doi: 10.3181/00379727-102-25342. [DOI] [PubMed] [Google Scholar]
  20. Tao T. W., Uhr J. W. Capacity of pepsin-digested antibody to inhibit antibody formation. Nature. 1966 Oct 8;212(5058):208–209. doi: 10.1038/212208a0. [DOI] [PubMed] [Google Scholar]
  21. UHR J. W., BAUMANN J. B. Antibody formation. I. The suppression of antibody formation by passively administered antibody. J Exp Med. 1961 May 1;113:935–957. doi: 10.1084/jem.113.5.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]

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