Figure 3.

The BDNF-induced release of NT-3 is mediated by p75. PC12 cells stably transfected with pBJ-5-NT-3 myc were washed twice, preincubated for 45 min in physiological buffer, and subsequently stimulated for 30 min at 37°C with high potassium buffer (KCl), physiological buffer (control), or purified neurotrophins at 100 ng/ml each in physiological buffer. The p75 blocking antibodies (REX) were used at a concentration of 20 μg/ml, whereas the Trk tyrosine kinase blocker K252a (K252a) was applied at a concentration of 100 nM. Experiments were performed in triplicate; error bars indicate ±SD.