Abstract
The following passive transfer experiments evaluated the contributions of the various host responses in recovery from mousepox. (a) Immune spleen cells transferred highly efficient antiviral activity, but preinfected recipients of these cells made no detectable splenic interferon or antibody in the 24 hr interval after cell transfer. (b) Passively administered interferon was ineffective. (c) Recipients of hyperimmune serum had much more antibody than recipients of immune spleen cells but significantly less antiviral activity. (d) Immune spleen cell populations with antiviral activity contained mediators of CMI to virus antigens. (e) The antiviral activity of immune spleen cells was specific; it was inhibited by in vitro treatment with ATS, anti-light chain serum, and anti-theta ascitic fluid, but not by removal of mononuclear phagocytes from the immune population. These results are interpreted to mean that recovery mechanisms conferred by immune spleen cells were triggered by specifically sensitized, thymus-derived lymphocytes, and that antibody and interferon responses were of less importance. A radiosensitive recipient component was necessary for the full expression of the antiviral activity of both immune cells and immune serum. It seemed likely that this component was the blood monocyte.
Full Text
The Full Text of this article is available as a PDF (832.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blanden R. V., Mackaness G. B., Collins F. M. Mechanisms of acquired resistance in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):585–600. doi: 10.1084/jem.124.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blanden R. V. Mechanisms of recovery from a generalized viral infection: mousepox. 3. Regression infectious foci. J Exp Med. 1971 May 1;133(5):1090–1104. doi: 10.1084/jem.133.5.1090. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blanden R. V. Mechanisms of recovery from a generalized viral infection: mousepox. I. The effects of anti-thymocyte serum. J Exp Med. 1970 Nov;132(5):1035–1054. doi: 10.1084/jem.132.5.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greaves M. F., Torrigiani G., Roitt I. M. Blocking of the lymphocyte receptor site for cell mediated hypersensitivity and transplantation reactions by anti-light chain sera. Nature. 1969 May 31;222(5196):885–886. doi: 10.1038/222885a0. [DOI] [PubMed] [Google Scholar]
- Lidén S. The mononuclear-cell infiltrate in allergic contact dermatitis. 2. Selective accumulation of cells from the bone marrow. Acta Pathol Microbiol Scand. 1967;70(1):58–66. doi: 10.1111/j.1699-0463.1967.tb01270.x. [DOI] [PubMed] [Google Scholar]
- Lubaroff D. M., Waksman B. H. Bone marrow as a source of cells in reactions of cellular hypersensitivity. I. Passive transfer of tuberculin sensitivity in syngeneic systems. J Exp Med. 1968 Dec 1;128(6):1425–1435. doi: 10.1084/jem.128.6.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mackaness G. B., Hill W. C. The effect of anti-lymphocyte globulin on cell-mediated reistance to infection. J Exp Med. 1969 May 1;129(5):993–1012. doi: 10.1084/jem.129.5.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mackaness G. B. The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969 May 1;129(5):973–992. doi: 10.1084/jem.129.5.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mason S., Warner N. L. The immunoglobulin nature of the antigen recognition site on cells mediating transplantation immunity and delayed hypersentivity. J Immunol. 1970 Mar;104(3):762–765. [PubMed] [Google Scholar]
- North R. J. The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. J Exp Med. 1970 Sep 1;132(3):521–534. doi: 10.1084/jem.132.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nossal G. J., Cunningham A., Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. 3. Chromosomal marker analysis of single antibody-forming cells in reconstituted, irradiated, or thymectomized mice. J Exp Med. 1968 Oct 1;128(4):839–853. doi: 10.1084/jem.128.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROBERTS J. A. ENHANCEMENT OF THE VIRULENCE OF ATTENUATED ECTROMELIA VIRUS IN MICE MAINTAINED IN A COLD ENVIRONMENT. Aust J Exp Biol Med Sci. 1964 Dec;42:657–666. doi: 10.1038/icb.1964.63. [DOI] [PubMed] [Google Scholar]
- Subrahmanyan T. P., Mims C. A. Fate of intravenously administered interferon and the distribution of interferon during virus infections in mice. Br J Exp Pathol. 1966 Apr;47(2):168–176. [PMC free article] [PubMed] [Google Scholar]
- Tripathy S. P., Mackaness G. B. The effect of cytotoxic agents on the passive transfer of cell-mediated immunity. J Exp Med. 1969 Jul 1;130(1):17–30. doi: 10.1084/jem.130.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Volkman A., Collins F. M. Recovery of delayed-type hypersensitivity in mice following suppressive doses of X-radiation. J Immunol. 1968 Nov;101(5):846–859. [PubMed] [Google Scholar]
- Volkman A. The origin and turnover of mononuclear cells in peritoneal exudates in rats. J Exp Med. 1966 Aug 1;124(2):241–254. doi: 10.1084/jem.124.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams R. M., Waksman B. H. Thymus-derived cells in the early phase of delayed tuberculin reactions. J Immunol. 1969 Dec;103(6):1435–1437. [PubMed] [Google Scholar]
- van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]