Figure 1.

(A) The two-step mating scheme used to produce SOD1^G85R, NF-L −/− mice. (B) Immunoblot of 20-μg spinal cord extracts made by using an antibody that recognizes mouse and human SOD1^G85R with equal affinity. (C) Parallel Coomassie blue-stained gel demonstrating comparable protein loading. (D and E) Coomassie blue-stained SDS/PAGE gel (D) and immunoblots (E) to measure NF-H and NF-L in 20 μg of sciatic nerve or spinal cord from wild-type mice and heterozygous NF-L and homozygous NF-L knockout mice.