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The relative level of GnRH gene expression changes spontaneously over a 16-h period. Quantification of photonic emissions were performed on GT1–1 cells transfected 24 h earlier. Counts were accumulated in two 30-min windows separated by 16 h. Values for the same cell at 0 h and 16 h are connected by a line. Data are derived from 20 individual cells studied in 12 independent experiments.
