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The functional half-life of luciferase in GT1–1 cells is reasonably short. Individual GT1–1 neurons were microinjected with purified firefly luciferase protein (1 μg/μl) and were subjected immediately to successive photonic measurements in the presence of luciferin (0.1 mM). Photonic signals were accumulated in 10-min bins. Results illustrate averaged photonic emissions (mean ± SEM) of 18 cells in a single experiment that is representative of three others.
