Abstract
A series of human cell lines has been examined for fibrinolysis in culture. The sera that are activating for fibrinolysis by human cells are mouse, monkey, human, horse, and bovine. Individual human sera show considerable variation in the ability to activate fibrinolysis. In common with other neoplastic or transformed mammalian and avian cell cultures, human cell lines of neoplastic origin produce substantial amounts of plasminogen activator. Several cultures of nonmalignant origin also produce plasminogen activator, whereas cultures obtained from trypsinized human embryos, or from human embryonic skin do not. The human melanoma plasminogen activators are of two kinds: a major component with a mol wt of 50,000, and a minor species with a mol wt of approximately 60,000. Both are DFP sensitive, serine proteases.
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Selected References
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- Astrup T. Tissue activators of plasminogen. Fed Proc. 1966 Jan-Feb;25(1):42–51. [PubMed] [Google Scholar]
- Bernik M. B. Increased plasminogen activator (urokinase) in tissue culture after fibrin deposition. J Clin Invest. 1973 Apr;52(4):823–834. doi: 10.1172/JCI107246. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bernik M. B., Kwaan H. C. Origin of fibrinolytic activity in cultures of the human kidney. J Lab Clin Med. 1967 Oct;70(4):650–661. [PubMed] [Google Scholar]
- Bernik M. B., Kwaan H. C. Plasminogen activator activity in cultures from human tissues. An immunological and histochemical study. J Clin Invest. 1969 Sep;48(9):1740–1753. doi: 10.1172/JCI106140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deykin D. The clinical challenge of disseminated intravascular coagulation. N Engl J Med. 1970 Sep 17;283(12):636–644. doi: 10.1056/NEJM197009172831207. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Ossowski L., Unkeless J. C., Tobia A., Quigley J. P., Rifkin D. B., Reich E. An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. II. Mammalian fibroblast cultures transformed by DNA and RNA tumor viruses. J Exp Med. 1973 Jan 1;137(1):112–126. doi: 10.1084/jem.137.1.112. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rifkin D. B., Reich E. Selective lysis of cells transformed by Rous Sarcoma virus. Virology. 1971 Jul;45(1):172–181. doi: 10.1016/0042-6822(71)90124-3. [DOI] [PubMed] [Google Scholar]
- TAGNON H. J., SCHULMAN P., WHITMORE W. F., LEONE L. A. Prostatic fibrinolysin; study of a case illustrating role in hemorrhagic diathesis of cancer of the prostate. Am J Med. 1953 Dec;15(6):875–884. doi: 10.1016/0002-9343(53)90178-8. [DOI] [PubMed] [Google Scholar]
- Unkeless J. C., Tobia A., Ossowski L., Quigley J. P., Rifkin D. B., Reich E. An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses. J Exp Med. 1973 Jan 1;137(1):85–111. doi: 10.1084/jem.137.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]