Table I.
Yeast Strains Used in This Study
| Strain | Genotype | Source | ||
|---|---|---|---|---|
| DDY182 | MAT a ura3-52 | 1 | ||
| DDY1132 | MAT a/α ura3-52/ura3-52 | 1 | ||
| R757 | MAT α ura3-52, his4-15, lys9 | 2 | ||
| R2563 | MAT α ura3-52, his4-15, lys9, Δerg6 | 2 | ||
| DDY186 | MAT α ura3-52, leu2-3,112, | 1 | ||
| DDY321 | MAT a his3-200, leu2-3,112, ura3-52, Δabp1::LEU2 | 1 | ||
| DDY332 | MAT a, ura3-52, his3-Δ200, Δsla1-Δ1::URA3 | 1 | ||
| DDY544 | MAT α ura3-52, ade2, leu2-3,112, Δsla2-Δ1::URA3 | 1 | ||
| DDY771 | MAT α, his4 ura3-52, Δsac6::URA3 | 1 | ||
| RH144-3D | MAT a, ura3-52, leu2-3,112, his4, bar1-1 | 3 | ||
| RH266-1D | MAT a, ura3-52, leu2-3,112, his4, bar1-1, end3-1 | 3 | ||
| DDY 816 | MAT α, his3-200, leu2-3,112, ura3-52 | 1 | ||
| DDY 817 | MAT α, his3-200, leu2-3,112, ura3-52, Δsrv2/CAP::HIS3 | 1 | ||
| DDY199 | MAT a his3, leu2, ura3, ade2, ade3, can1, sap3 | 4 | ||
| DDY200 | MAT a his3, leu2, ura2, ade3, can1, sap3, Δtpm1::URA3 | 4 | ||
| YJC093 | MAT a rho +, his3-11, leu2-3,112, trp1-1, ura3-1 | 5 | ||
| YJC108 | MAT a rho +, his3-11, leu2-3,112, trp1-1, ura3-1, cap2-Δ1::HIS3 | 5 | ||
| DDY280 | MATα ura3-62, leu2-3,112, trp1-1, his4-619, bem1Δ::LEU2 | 6 | ||
| DDY1157 | MATa/α ura3-52/ura3-52, Δsec8::URA3/ Δsec8::URA3, leu2,3-112::LEU2-Sec8-myc/ leu2-3,112:LEU2-Sec8-myc | 7 | ||
| JZ414 | MAT a/α ura3-52/ura3-52, his3Δ200/ his3Δ200, leu2-Δ1/leu2-Δl lys2-801. trp1-Δ1/trp1-Δ1 + pBUD6, 2μ URA3 | 8 | ||
| M-442 | MATa/α ura3-52/ura3-52, his3Δ200/ his3Δ200, leu2-Δ1/leu2-Δ1 lys2-801/lys2-801, trp1-Δ1/trp1-Δ1, gin4Δ::HIS3/gin4Δ::HIS3, GIN4-GST::URA3/GIN4-GST::URA3 | 8 | ||
| *DDY336 | MAT a, act1-133::HIS3, can1-1, cry1 | 1 | ||
| *DDY337 | MAT a, act1-108::HIS3, can1-1, cry1 | 1 | ||
| *DDY338 | MAT a, act1-101::HIS3, can1-1, cry1 | 1 | ||
| *DDY339 | MAT a, act1-102::HIS3, ade2-101, can1-1 | 1 | ||
| *DDY340 | MAT α, act1-104::HIS3, can1-1 | 1 | ||
| *DDY341 | MAT a, act1-111::HIS3, ade2-101, cry1 | 1 | ||
| *DDY342 | MAT a, act1-113::HIS3, can1-1 | 1 | ||
| *DDY343 | MAT α, act1-115::HIS3, ade2-201, can1-1 | 1 | ||
| *DDY344 | MAT α, act1-116::HIS3, can1-1 | 1 | ||
| *DDY345 | MAT a, act1-117::HIS3, ade2-101, cry1 | 1 | ||
| *DDY346 | MAT a, act1-119::HIS3, ade2-101, cry1 | 1 | ||
| *DDY347 | MAT a, act1-120::HIS3, can1-1, cry1 | 1 | ||
| *DDY348 | MAT a, act1-123::HIS3, ade2-101, can1-1, cry1 | 1 | ||
| *DDY349 | MAT α, act1-124::HIS3 | 1 | ||
| *DDY350 | MAT α, act1-125::HIS3 | 1 | ||
| *DDY351 | MAT α, act1-129::HIS3, can1-1 | 1 | ||
| *DDY352 | MAT a, act1-132::HIS3, ade2-101, can1-1, cry1 | 1 | ||
| *DDY353 | MAT α, act1-135::HIS3 | 1 | ||
| *DDY354 | MAT a, ACT1::HIS3, cry1 | 1 | ||
| *DDY355 | MAT a, act1-112::HIS3, ade2-101, ade4, cry1 | 1 | ||
| *DDY356 | MAT α, act1-105::HIS3, can1-1 | 1 | ||
| *DDY357 | MAT a, act1-136::HIS3, | 1 | ||
| *DDY654 | MAT α, act1-121::HIS3, can1-1 | 1 | ||
| 972 | h- wild-type Schizosaccharomyces pombe | 9 |
*
These strains are a congenic collection of act1 alleles made as described by Wertman et al. (1992). Only alleles that vary between strains are listed above. All strains are his3-Δ200, ura3-52, leu2-3,112, and tub2-201. Sources of strains listed. (1). This lab; (2) R. Gaber (Northwestern University, Evanston, IL); (3) H. Riezman (Biozentrum, University of Basel, Switzerland); (4) A. Bretscher (Cornell University, Ithaca, NY); (5) J. Cooper (Washington University, St. Louis, MO); (6) I. Herskowitz (UCSF, San Francisco, CA); (7) Dan TerBush, Peter Novick (Yale University School of Medicine, New Haven, CT); (8) J. Pringle (University of North Carolina, Chapel Hill, NC); (9) Paul Nurse (ICRF, London, UK).