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. 1997 Apr 21;137(2):291–303. doi: 10.1083/jcb.137.2.291

Figure 5.

Figure 5

Figure 5

Transcripts from the 93D region and detection of the locus by oligonucleotide hybridization. (A) Schematic representation of the transcripts from the heat shock RNA-ω region in 93D. The gray box indicates the location of the intron which is spliced out of the smaller ω-c RNA. The repeat region of the long ω-n transcript is shown in black. The sequence of the fluorescently labeled oligonucleotide, Fl-P2, used as a probe for ω-n is given below a 29 base transcript sequence which is strongly conserved between the tandem repeats. B and C demonstrate that the probe specifically binds to 93D RNA in squashed polytene chromosomes. (B) DNA stained with DAPI. (C) 93D hybridized with FlP2 under nondenaturing conditions.