Table I.
The Effect of Inhibiting MAP Kinase Activation on the Spindle Assembly Checkpoint: Summary of Microinjection Data
| Treatment | Outcome | |||||
|---|---|---|---|---|---|---|
| Remained arrested in mitosis through 4 h | Mitosis completed within 4 h | Progressed to interphase within 4 h without completing mitosis | ||||
| Nocodazole plus buffer injection | 10/10 | 0/10 | 0/10 | |||
| XCL100 injection | 0/20 | 20/20 | 0/20 | |||
| Nocodazole plus XCL100‡ | 0/15‡ | 0/15‡ | 15/15‡ | |||
| Nocodazole plus XCL100 C260S | 17/17 | 0/17 | 0/17 | |||
| MKK-1* injection | 0/19 | 19/19 | 0/19 | |||
| Nocodazole plus XCL100 plus MKK-1* | 8/8 | 0/8 | 0/8 | |||
‡
The data tabulated here are taken from four independent microinjection experiments using four separate preparations of XCL100. Three other preparations of XCL100 showed no effect (cells remained arrested in mitosis for 4 h after nocodazole treatment) when microinjected into a total of about a dozen mitotic cells.