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. 1997 Apr 21;137(2):359–375. doi: 10.1083/jcb.137.2.359

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Effect of antisense oligonucleotides on the polarity of sucrase isomaltase in CACO-2 cells. CACO- 2 C2BBe1 monolayers were grown and incubated with random (a), random/2 (c), A19 (b), or A19/2 (d) oligonucleotides as described before. The cells were fixed in 3% PFA and processed for indirect immunofluorescence with a mAb anti-sucrase isomaltase. The monolayers were analyzed under a confocal microscope, and three-dimensional reconstructions in a plane perpendicular to the monolayer were obtained from stacks of confocal optical sections. Bars, 10 μm.

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