Table II.

Apical Expression of Villin in CACO-2 Cells Treated with A19 Oligonucleotide

		Cells with microvillum images of villin		Cells without apical image of villin
		%		%
CK19+		23		33
CK19−		6		38

CACO-2 cells treated with A19 oligonucleotide as described before were sequentially processed for double immunofluorescence with anti-villin mAb and biotinylated antiCK19 mAb (RCK108; see Materials and Methods). CK19+ refers to cells with CK19. Cells with lower amounts of CK19 but still showing IF were counted in this category. CK19− cells were those with no detectable CK19.