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. 1997 Apr 21;137(2):319–333. doi: 10.1083/jcb.137.2.319

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Effect of microinjected anti-EAGE on BFA induced relocation of Golgi enzymes. Vero cells were microinjected with Fabfragments of anti-EAGE. 30 min after injection 5 μg/ml BFA was added to the medium and cells were incubated for further 2.5 h at 37°C. Cells were then fixed and double stained for injected antibodies (b, d, and f) and marker proteins for (a) the ER (PDI), (c) the intermediate compartment (ERGIC 53), and (e) the Golgi complex (NAGT1). Microinjected antibodies accumulate in patches (arrowheads in d; arrows in f) which colocalize with NAGT1 (arrows in e) but not ERGIC 53 and PDI. The injected anti-EAGE thus affect the BFA-induced relocalization of Golgi proteins to the ER. Bar, 10 μm.