Table II.
Comparison of the Properties of the p62cplx-associated PI3-kinase with Other Described PI3-kinases
| p62cplx-associated | Vps34p‡ | Human PI3-kinase* (VPS34 homologue) | PI3-kinase* (p110) | |||||
|---|---|---|---|---|---|---|---|---|
| Adaptor molecule | p62 | Vps15p | p150 | p85 | ||||
| Wortmannin (IC50) | 3.5 μM | 3 μM | 2.5 nM | 3 nM | ||||
| Affect of low NP-40 (0.1–0.5%) | Stimulation | Stimulation | Inhibition | Stimulation | ||||
| NP-40 (IC50) | 0.35% | 1.0% | 0.01% | 0.1% | ||||
| Adenosine sensitivity | No | No | No | No | ||||
| PI (4)P as substrate | No | No | No | Yes | ||||
| Cation preference | Mn2 > Mg2+ | Mn2+ = Mg2+ | Mn2+ | Mg2+ = Mn2+ |
Our data for the p62cplx-associated activity is summarized. Data for the other PI3-kinases is from the references noted. Only the well-characterized PI3-kinases have been included in this table.
*
Data from Volinia et al., 1995.
‡
Data from Stack and Emr, 1995.