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. 1997 Oct 20;139(2):313–325. doi: 10.1083/jcb.139.2.313

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(A) Effect of RCC1 depletion on Rch1 recycling. tsBN2 cells were injected with Rch1_33–529 into the cytoplasm followed by incubation at 33.5°C (a and b) or 39.5°C (c and d). Fluorescence micrographs show the localization of Rch1 in cells that were fixed after 0 min (a and c) or 4 h (b and d) of incubation under the conditions indicated. (B) Time course of nuclear accumulation of cytoplasmically injected Rch1_33–529 in the absence and presence of RCC1. tsBN2 and BHK21 cells were injected into the cytoplasm with Rch1_33–529 and incubated at 39.5°C or, as a control, at 33.5°C for various time periods, fixed and stained. Quantification was done as in Fig. 1 B and the percentage of Rch1 in the nucleus was expressed as a function of time after injection. Bar, 25 μm.

(A) Effect of RCC1 depletion on Rch1 recycling. tsBN2 cells were injected with Rch1_33–529 into the cytoplasm followed by incubation at 33.5°C (a and b) or 39.5°C (c and d). Fluorescence micrographs show the localization of Rch1 in cells that were fixed after 0 min (a and c) or 4 h (b and d) of incubation under the conditions indicated. (B) Time course of nuclear accumulation of cytoplasmically injected Rch1_33–529 in the absence and presence of RCC1. tsBN2 and BHK21 cells were injected into the cytoplasm with Rch1_33–529 and incubated at 39.5°C or, as a control, at 33.5°C for various time periods, fixed and stained. Quantification was done as in Fig. 1 B and the percentage of Rch1 in the nucleus was expressed as a function of time after injection. Bar, 25 μm.