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. 1997 Oct 20;139(2):417–434. doi: 10.1083/jcb.139.2.417

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MT breakage induced by local MT buckling in the lamella. Selected images from series of fluorescence micrographs of cells injected with X-rhodamine–labeled tubulin. Time in min/ sec is shown in the upper left of each panel. (A) A MT with local buckling broke at 11 s. The newly formed minus end (large arrowhead) shortened immediately after breakage, while the new plus end (small arrowhead) formed by the break shortened slightly before beginning to undergo dynamic instability (times 00:19–1:57). (B) A MT with local buckles broke (time = 00:10), forming a new minus end (large arrowhead) that remained stable and a new plus end that shortened before undergoing dynamic instability (times 00:20–01:59). Bar, 10 μm.