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. 1997 Oct 6;139(1):205–217. doi: 10.1083/jcb.139.1.205

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p35 does not block cell death. After 5 d in vitro, granule cells were transfected, as described in Materials and Methods, with pGreen Lantern-1 and either pBluescriptII, a BCL-2 construct, or a p35 construct. At 7 d in vitro, the number of pGreen Lantern-1–positive (fluorescent) cells was determined before switching cells to K25+S or K5+S medium. The number of pGreen Lantern-1–positive cells was again determined after 24 and 48 h and compared to the original number of pGreen Lantern-1–positive cells before the medium was switched. Data represent mean ± SEM for four independent experiments. *indicates significance at P < 0.05 in a paired t test in which the data passed a test for normality.