Figure 2.
Bax-deficient granule cells are fully protected from K+ or K+/serum deprivation-induced death. After 7 d in vitro, granule cells were switched to either K5+S (open circle, square, and triangle) or K5-S medium (closed circle, square, and triangle) for 12, 24, 48, or 72 h. Survival was assessed by counting neurons in photomicrographs of calcein AM-stained cultures and compared to cultures maintained in K25+S. Circles, squares, and triangles represent Bax +/+, Bax +/−, and Bax −/− cultures, respectively. Data represent mean ± range for two independent experiments.
