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. 1997 Oct 6;139(1):205–217. doi: 10.1083/jcb.139.1.205

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Inhibitors of caspases do not block death, but do block DEVD-AMC cleavage. Cultures were switched to K5+S, K5+S plus 100 μM BAF, or K5+S plus 100 μM ZVAD-FMK. Control cultures were switched to K25+S medium. (A) After 12, 24, or 48 h neuronal survival was determined by calcein AM staining. (mean ± SD, N = 3 experiments) (B) After 8, 12, 18, 24, or 48 h cultures were lysed and assayed for DEVD-AMC cleavage. Fluorescence was measured after 20 min at room temperature (mean ± range, N = 2 experiments).