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. 1997 Oct 6;139(1):49–61. doi: 10.1083/jcb.139.1.49

Figure 6.

Figure 6

Low pH buffer removes surface-bound Tac antibodies, but not the internalized Tac antibodies in the tubular compartment. Tac- and ARF6-transfected HeLa cells were chilled to 4°C and incubated with mouse anti-Tac antibodies for 30 min. One set of cells were fixed at 4°C either before (4 °C Total) or after (4 °C Internal) rinsing with low pH buffer (0.5% acetic acid, 0.5 M NaCl, pH 3.0). Another set of cells were warmed to 37°C for 30 min in the presence of 1 μM CD and then fixed before (37 °C + CD Total) or after (37 °C +CD Internal) rinsing with low pH buffer. Fixed cells were then processed for indirect immunofluorescence.