Table I.

E-selectin Expression Is Not Stimulated by Supernatant from Migrating Endothelial Cells

Addition		MFI
Unwounded SN		0.01 ± 0.01
6 h SN		0.04 ± 0.04
24 h SN		0.02 ± 0.02
TNF (10 U/ml)		20.5 ± 5.9

A confluent EC monolayer was multiply wounded such that all cells in the well were induced to migrate. The cells were washed twice to remove debris and replaced with fresh medium. Supernatants (SNs) were collected at 6 and 24 h thereafter and added to other wells containing confluent EC. TNF (10 U/ml) was added to some of these wells as a control, and E-selectin expression measured 4 h later. The results are given as the MFI ± SEM of two experiments. Neither the 6 nor the 24 SN gave significant increases in E-selectin expression compared to SN from unwounded cells.