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. 1997 Oct 6;139(1):193–204. doi: 10.1083/jcb.139.1.193

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Western blot analysis of synaptopodin with mAb G1. Lanes 1–4 show the immunodetection; lanes 1′–4′ show staining of the identical membrane with Coomassie brilliant blue to demonstrate protein loading and separation. Cytosolic extracts from rat forebrain (lane 1), cerebellum (lane 2), and isolated glomeruli (lanes 3 and 4) were analyzed in parallel. While in the forebrain a protein of ∼100 kD is present, no protein expression is seen in cerebellum. In kidney a 110-kD band is observed. The extract in lane 4 had been kept at 4°C for 24 h before separation. Several proteolytic fragments appear; the band indicated by an arrowhead represents the originally described 44-kD protein.