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. 1997 Oct 6;139(1):193–204. doi: 10.1083/jcb.139.1.193

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Expression of synaptopodin in cultured hippocampal neurons. (a) In 25-d-old cultured hippocampal neurons, a dotted pattern of immunoreactivity in dendrites is seen, whereas axons (arrows) are not labeled. (b) Phase contrast microscopy. (c) Confocal laser scanning double fluorescence analysis of synaptopodin (red) and MAP2 (green) to demonstrate the exclusive expression of synaptopodin in dendrites. (d) Confocal laser scanning double fluorescence analysis of synaptopodin and synaptophysin. The yellow signal results from a complete overlap of the immunoreactivity and proves the synaptic localization of synaptopodin in cultured neurons. Bar, 10 μm.