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. 1997 Oct 6;139(1):295–307. doi: 10.1083/jcb.139.1.295

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Effects of anti-EDA mAbs and recombinant EDA fragments on cell spreading mediated by recombinant FNs. (A) Microtiter plates precoated with 5 μg/ml of rFN(C) (open bars) or rFN(AC) (closed bars) were treated with the following mAbs (20 μg/ml) at 37°C for 30 min before the addition of HT1080 cells: None, no addition of mAbs; IST-9 and HHS-01, two different anti-EDA mAbs; FN12-8+FN30-8, a mixture of two function-blocking mAbs directed to CCBD. The cells were incubated at 37°C for 30 min and the number of cells adopting a well spread morphology was determined. (B) In separate experiments, HT1080 cells were seeded onto microtiter plates precoated with 5 μg/ml of rFN(C) (open bars) or rFN(AC) (closed bars) and incubated for 30 min at 37°C in the presence or absence of MBP fusion fragments with (MBP11-A-12) and without (MBP-11-12) the EDA segment. Each bar represents the mean ± SD (n = 6).

Effects of anti-EDA mAbs and recombinant EDA fragments on cell spreading mediated by recombinant FNs. (A) Microtiter plates precoated with 5 μg/ml of rFN(C) (open bars) or rFN(AC) (closed bars) were treated with the following mAbs (20 μg/ml) at 37°C for 30 min before the addition of HT1080 cells: None, no addition of mAbs; IST-9 and HHS-01, two different anti-EDA mAbs; FN12-8+FN30-8, a mixture of two function-blocking mAbs directed to CCBD. The cells were incubated at 37°C for 30 min and the number of cells adopting a well spread morphology was determined. (B) In separate experiments, HT1080 cells were seeded onto microtiter plates precoated with 5 μg/ml of rFN(C) (open bars) or rFN(AC) (closed bars) and incubated for 30 min at 37°C in the presence or absence of MBP fusion fragments with (MBP11-A-12) and without (MBP-11-12) the EDA segment. Each bar represents the mean ± SD (n = 6).