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. 1997 Oct 6;139(1):295–307. doi: 10.1083/jcb.139.1.295

Figure 7.

Figure 7

Effect of glycosidase treatments on spreading of HT1080 cells on recombinant FNs. HT1080 cells were treated with 0.1 U/ml of heparitinase I, heparinase, or chondroitinase ABC for 30 min at 37°C. Glycosidase-treated cells were added to microtiter plates precoated with 5 μg/ml of rFN(C) (open bars) or rFN(AC) (closed bars) and incubated for 30 min at 37°C. Cell spreading was quantified as described in Materials and Methods. Each bar represents the mean ± SD (n = 6).