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. 1997 Oct 6;139(1):265–278. doi: 10.1083/jcb.139.1.265

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Localization of vWF and lacZ expression in normal (+/+) and β1-null (−/−) teratomas. Teratomas were removed 21 d after inoculation of ES cells and sectioned. Sections were stained overnight for lacZ expression and subsequently immunostained for vWF expression. In normal teratomas, vWF-positive areas (A, arrows) are found to be positive and negative for lacZ expression (C, corresponding arrows) indicating that endothelial cells are both host- and ES cell–derived. In contrast, in β1-null teratomas, all vWF-positive areas (B, arrows) are lacZ-negative (D, corresponding arrows) indicating that endothelial cells are solely host derived. Many other areas, however, that are vWF-negative (B, arrowhead) are clearly lacZ-positive (D, corresponding arrowhead) indicating that many other regions in β1-null teratomas are ES cell–derived. Bar, 100 μm.

Localization of vWF and lacZ expression in normal (+/+) and β1-null (−/−) teratomas. Teratomas were removed 21 d after inoculation of ES cells and sectioned. Sections were stained overnight for lacZ expression and subsequently immunostained for vWF expression. In normal teratomas, vWF-positive areas (A, arrows) are found to be positive and negative for lacZ expression (C, corresponding arrows) indicating that endothelial cells are both host- and ES cell–derived. In contrast, in β1-null teratomas, all vWF-positive areas (B, arrows) are lacZ-negative (D, corresponding arrows) indicating that endothelial cells are solely host derived. Many other areas, however, that are vWF-negative (B, arrowhead) are clearly lacZ-positive (D, corresponding arrowhead) indicating that many other regions in β1-null teratomas are ES cell–derived. Bar, 100 μm.