Table I.

Quantification of Branches of Blood Vessels in Normal (+/+) and β1-null (−/−) Embryoid Bodies Treated Without or With VEGF

		Without VEGF		10 ng/ml VEGF		20 ng/ml VEGF
(1/1)		273 ± 103 (n = 9)		363 ± 103 (n = 8)		372 ± 86 (n = 7)
(2/2)		41 ± 30 (n = 10)		32 ± 20 (n = 8)		76 ± 85 (n = 7)

Embryoid bodies were cultured 5 d in suspension and 12 d on gelatinized glass cover slips in the absence or presence of 10 or 20 ng/ml VEGF, respectively. After the end of the culture period, embryoid bodies were fixed and stained for PECAM expression. Data show all vessel branches per embryoid body and are expressed in mean + SD. n is number of embryoid bodies analyzed.