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. 1997 Oct 6;139(1):23–36. doi: 10.1083/jcb.139.1.23

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Soi1p is a high molecular weight protein that is found in a detergent-insensitive, sedimentable fraction. Labeled cell extracts were produced and processed as described in Materials and Methods. After a clearing spin, lysates were spun at ∼13,000 g to produce a pellet (P13). Triton X-100 (0.1%) was added to half of the supernatant fraction from this spin and the detergent-treated and untreated samples were then centrifuged at ∼150,000 g to produce a pellet (P150) and a supernatant fraction (S150). Kex2p and Soi1p-HA were immunoprecipitated from SDS-denatured samples and were separated by SDS-PAGE (see Materials and Methods).