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Immunoblot analysis of Bni4p-specific antibodies. Extracts of bni4-Δ1/bni4-Δ1 strain DDY175 (lane 1); wild-type strain YEF473 (lane 2); and strain YEF473 containing the high-copy BNI4 plasmid p356 (lane 3) were analyzed using affinity-purified antibodies to Bni4p (see Materials and Methods). The mobilities of molecular mass markers are indicated.
