Table III.

Oligonucleotide Primers Used for PCR-mediated Gene Deletion and Cloning

Name of primer		Sequence of primer
CHS4-5′-TRP1*		CGTCTTTTTTGGTGATAAAGTTAAAATAAAAGGATAAAAAGTACAATCTTGATCCGGAGC
CHS4-3′-TRP1*		GTTGCACCTATAAAGAATGAAAACAATCTAGTATGTGTACCTCCTTACGCATCTGTGCGG
CDC10-5′-HIS3*		CAAGCCCCACGGTTACTACAAGCACTGTATAAATATATTAGATTGTACTGAGAGTGCACC
CDC10-3′-HIS3*		AGGAGAGAAGCAAGTTACGGTCTTTTCATATAGAATAATTCTGTGCGGTATTTCACACCG
CHS3-5′-HIS3*		GGTCCTGTTTAGACTATCCGCAGGAAAGAAATTAGAAAGAGCTTTGGTGAGCGCT
CHS3-3′-HIS3*		GTAAGTATCACAGTAAAAATATTTTCATACTGTCTATCCGTCGAGTTCAAGAGA
BNI4-5′-TRP1*		ATGTCGGATAGTATTTCAGATTCAAAGTCCTCAGAACTTTGTACAATCTTGATCCGGAGC
BNI4-3′-TRP1*		ATTTGATTCATTTCCATTTCTCCCAGTTTTCTGCTCTAATCTCCTTACGCATCTGTGCGG
CHS4-5′‡		CGCGGATCCAAATGGCAAGTTCACCGC
CHS4-3′‡		CGCGGATCCCAGTCCAAAAACCCCAG
CHS4-3′C693S§		ACAATCCTCGAGGTGTACTTACATAATTACAGAGTCTTTTTTTTTTTTACCTTG
CHS3F5′‖		CGGCGGCCATGGATGGGCAAGTTCACCGCAGGTACATCC
CHS3MR¶		GGGCTCGAGCGTTGCCCGAGCCCTTAATTAAACCATCACAAAC

^* Primers used to generate deletion cassettes for the indicated genes. Nucleotides corresponding to the flanking regions of the gene to be deleted are in bold face; the remaining nucleotides correspond to regions upstream and downstream of the selectable marker gene.  

^‡ BamHI sites and the first ATG of CHS4 are underlined.  

^§ A XhoI site and the missense mutation that substitutes serine for cysteine are underlined.  

^‖A NcoI site and the first ATG of CHS3 are underlined.  

^¶ A XhoI site is underlined.