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. 1997 May 19;137(4):939–952. doi: 10.1083/jcb.137.4.939

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Differentiation in the nonproliferating centers of cell colonies. (A) Photomicrographs of colonies arising from cells seeded at clonal density and grown for 10 d in GM. Representative parental (P) and v-Myc+ (M) colonies, showing the presence of myotubes in the centers of only v-Myc+ colonies. (B) Lower magnification of plates from (A). (C) The percentage of colonies showing myotubes. Colonies arising after 10-d incubations in GM were scored for the presence of myotubes. Average results for two, and for some lines three, experiments are shown. The plating efficiencies for colony formation varied from 50 to 92% between individual experiments and varied no more than 10% between cell lines in a given experiment. Bar: (A) 0.4 mm; (B) 1.7 mm.

Differentiation in the nonproliferating centers of cell colonies. (A) Photomicrographs of colonies arising from cells seeded at clonal density and grown for 10 d in GM. Representative parental (P) and v-Myc+ (M) colonies, showing the presence of myotubes in the centers of only v-Myc+ colonies. (B) Lower magnification of plates from (A). (C) The percentage of colonies showing myotubes. Colonies arising after 10-d incubations in GM were scored for the presence of myotubes. Average results for two, and for some lines three, experiments are shown. The plating efficiencies for colony formation varied from 50 to 92% between individual experiments and varied no more than 10% between cell lines in a given experiment. Bar: (A) 0.4 mm; (B) 1.7 mm.