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. 1997 May 19;137(4):939–952. doi: 10.1083/jcb.137.4.939

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CEA transfectants of L6 and C2 myoblasts retain division potential in DM. FACS profiles (A) of parental rat L6 cells (P), CEA transfectant clone (C-1) and CEA total transfectant population (C), and (C) mouse C2 and CEA transfectant total population (C2C) labeled with rabbit anti–human CEA antibodies. In all cases, anti-CEA immunoreactivity was detected using goat anti–rabbit FITC-conjugated antibody, as described in Materials and Methods. (B) L6 and (D) C2 cells expressing CEA retain division potential in DM, as measured by their ability to form colonies in GM. Cells were seeded in GM on day −3, and the medium was changed to DM on day 0. To calculate percentage viability, colony numbers were normalized to values obtained on day 2 in GM (day −1 in DM). Fusion indices are shown by dotted lines.