Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 May 19;137(4):813–824. doi: 10.1083/jcb.137.4.813

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Coimmunoprecipitation of Lhs1p with pro-CPY. (A and B) H660 (lhs1⁻ c-myc–LHS1 ⁺ sec18) cells were incubated as in Fig. 5 for 1 h at 37°C (lanes 1 and 5), 20 min at 50°C (lanes 2 and 6), and at 24°C with CHX for 3 h (lanes 3 and 7) or 6 h (lanes 4 and 8). After lysis of the cells under nondenaturing conditions, the samples were divided in two. One set of samples was immunoprecipitated (IP) with anti-CPY antiserum (α-CPY, A and B, lanes 1–4) and the other with preimmune serum (pim; A and B, lanes 5–8). Lanes 9 contained total lysate sample. After SDSPAGE and blotting, the proteins were immunostained (IS) using anti–c-myc antibody (α-c-myc; A, lanes 1–9) or anti-CPY antiserum (B, lanes 1–9). c-myc–Lhs1p (Lhs1p), pro-CPY (CPY), and IgG are indicated. Molecular mass markers (kD) are indicated on the left.