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. 1997 Apr 7;137(1):131–140. doi: 10.1083/jcb.137.1.131

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Epitope tagged wild-type human βMHC protein assembles into normal myofibrils in transiently transfected NRC. Cells were transfected with plasmids containing the wild-type human βMHC cDNA tagged with either the EE (A–C) or HA (D–F). The cells were stained for the presence of actin filaments using rhodamine–phalloidin (A and C) and were costained with the anti-EE (B) or anti-HA (E) specific antibodies. Sarcomeres containing human βMHC have well defined A bands (a), I bands (i), and M lines (m). The composite images (C and F) show that the exogenous βMHC (green) fills the A band, except the H zone as expected, in a pattern that is complementary and partially overlapping with F-actin (red, C and F). Bar, 20 μm (inset enlarged 3×).