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Expression of K184R βMHC in NRC disrupts myofibril assembly. NRC transfected with the human βMHC K184R plasmid were stained for with the anti-EE epitope specific antibody (B, D, and F). These cells were costained with anti-βMHC (A), anti-myomesin (C), or rhodamine–phalloidin (E). Sequential staining with two primary monoclonal antibodies is described in Materials and Methods. Bar, 20 μm.
