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Thick filaments containing R403Q have normal organization within the sarcomere. NRC transfected with human R403Q plasmid were costained for exogenous βMHC (B) with the epitope specific antibody and myomesin (A). The merged image (C) shows that myomesin staining is completely localized to the M-line, indicating normal thick filament and sarcomere structure. Bars: (A) 20 μm; (inset), 5 μm.
