Table I.
Morphology of Transfected NRC
| Transfected DNA* | Percentage of cells with organized myofibrils | |||||
|---|---|---|---|---|---|---|
| Epitope | Actin | Myomesin | ||||
| pCB6 | n.a.‡ | 40§ | 40§ | |||
| Wild-type‖ | 33 | 100 | 100 | |||
| K184R | 0 | 1.1 | 0.8 | |||
| R249Q | 32 | 100 | 100 | |||
| R403Q | 34 | 100 | 100 | |||
| S472V | 33 | 100 | 100 | |||
For all DNAs transfected, at least 2,000 cells were scored over several different transfected cultures.
n.a., not available.
Since transfected cells are not identifiable for the control backbone vector, pCD6, NRC were randomly scored for endogenous F-actin (via rhodamine–phalloidin) or myomesin (B4 antibody).
For the epitope tagged constructs only immunopositive cells were counted. The numbers are presented as percentage of transfected NRC, which display fully organized myofilaments as detected by indirect immunofluorescence. Epitope specific antibodies identify the exogenous βMHC protein. In transfected NRC with highly organized myofilaments, the percentage of highly ordered endogenous myofibril markers is also given.